ASTM E1532-00(2006)
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome

Standard No.

ASTM E1532-00(2006)

Release Date

2000

Published By

American Society for Testing and Materials (ASTM)

Status

Withdraw 2014-08

Latest

ASTM E1532-00(2006)

Scope

Mycoplasma hyorhinis, cultivar α strains (1)³ do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell cultures, are detected by indirect methods.

A specialized medium has been described but it is not yet in wide use (2).

This practice should be used in conjunction with Practice E 1531.

All cell cultures to be examined for mycoplasma should undergo a minimum of two passages in antibiototic-free tissue culture medium before testing.

1.1 This practice covers the use of cell cultures and DNA-binding flurorochrome techniques to detect mycoplasma contamination of cell cultures.

1.2 This practice does not cover axenic cultivation or identification of mycoplasmas.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E1532-00(2006) Referenced Document

• ASTM E1531 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium

ASTM E1532-00(2006) history

• 2000 ASTM E1532-00(2006) Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome
• 2000 ASTM E1532-00 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome