ASTM E1531-00(2006)
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium

Standard No.

ASTM E1531-00(2006)

Release Date

2000

Published By

American Society for Testing and Materials (ASTM)

Status

Withdraw 2014-08

Latest

ASTM E1531-00(2006)

Scope

1.1 This practice covers the procedures used for detection of mycoplasma contamination by direct microbiological culture.

1.2 This practice does not cover indirect methods for detection of mycoplasma such as DNA staining, biochemical detection, or genetic probes.

1.3 This practice does not cover methods for identification of mycoplasma organisms.

1.4 This practice will not detect cultivar strains (1) of Mycoplasma hyorhinis.

1.5 This practice is not intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E1531-00(2006) Referenced Document

• ASTM E1532 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome

ASTM E1531-00(2006) history

• 2000 ASTM E1531-00(2006) Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium
• 2000 ASTM E1531-00 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium