ASTM E1532-00
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome

Standard No.
ASTM E1532-00
Release Date
2000
Published By
American Society for Testing and Materials (ASTM)
Status
Replace By
ASTM E1532-00(2006)
Latest
ASTM E1532-00(2006)
Scope

1.1 This practice covers procedures used for the detection of mycoplasma contamination by DNA staining.

1.2 This practice does not cover cultivation methods for the detection of mycoplasma or other methods such as biochemical detection, hybridization methodology, histochemical stains, or immunochemical methods.

1.3 This practice does not cover methods for the identification of mycoplasma organisms.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E1532-00 Referenced Document

  • ASTM E1531 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium
  • ASTM E1533 Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4`-6-Diamidino-2-2 Phenylindole (DAPI) Staining
  • ASTM E1536 Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method

ASTM E1532-00 history

  • 2000 ASTM E1532-00(2006) Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome
  • 2000 ASTM E1532-00 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome
Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of the Bisbenzamide DNA-Binding Fluorochrome



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