1.1 This guide provides personnel who have a limited microbiological background with an understanding of the symptoms, occurrence, and consequences of chronic microbial contamination. The guide also suggests means for detection and control of microbial contamination in fuels and fuel systems. This guide applies primarily to gasoline, aviation, boiler, industrial gas turbine, diesel, marine, furnace fuels and blend stocks (see Specifications D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, and D6751), and fuel systems. However, the principles discussed herein also apply generally to crude oil and all liquid petroleum fuels. ASTM Manual 472 provides a more detailed treatment of the concepts introduced in this guide; it also provides a compilation of all of the standards referenced herein that are not found in the Annual Book of ASTM Standards, Section Five on Petroleum Products and Lubricants.
1.2 This guide is not a compilation of all of the concepts and terminology used by microbiologists, but it does provide a general understanding of microbial fuel contamination.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM D6469-20 Referenced Document
ASTM D1067 Standard Test Methods for Acidity or Alkalinity of Water
ASTM D1126 Standard Test Method for Hardness in Water
ASTM D1298 Standard Test Method for Density, Relative Density (Specific Gravity), or API Gravity of Crude Petroleum and Liquid Petroleum Products by Hydrometer Method
ASTM D130 Standard Test Method for Detection of Copper Corrosion from Petroleum Products by the Copper Strip Tarnish Test
ASTM D1331 Standard Test Methods for Surface and Interfacial Tension of Solutions of Surface-Active Agents
ASTM D1426 Standard Test Methods for Ammonia Nitrogen In Water
ASTM D1655 Standard Specification for Aviation Turbine Fuels
ASTM D1744 Standard Test Method for Determination of Water in Liquid Petroleum Products by Karl Fischer Reagent
ASTM D1976 Standard Test Method for Elements in Water by Inductively-Coupled Argon Plasma Atomic Emission Spectroscopy
ASTM D2068 Standard Test Method for Filter Blocking Tendency of Distillate Fuel Oils
ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D4057 Standard Practice for Manual Sampling of Petroleum and Petroleum Products
ASTM D4176 Standard Test Method for Free Water and Particulate Contamination in Distillate Fuels (Visual Inspection Procedures)
ASTM D4412 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
ASTM D4418 Standard Practice for Receipt, Storage, and Handling of Fuels for Gas Turbines
ASTM D445 Standard Test Method for Kinematic Viscosity of Transparent and Opaque Liquids (the Calculation of Dynamic Viscosity)
ASTM D4454 Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
ASTM D4814 Standard Specification for Automotive Spark-Ignition Engine Fuel
ASTM D4840 Standard Guide for Sample Chain-of-Custody Procedures
ASTM D4860 Standard Test Method for Free Water and Particulate Contamination in Mid-Distillate Fuels (Clear and Bright Numerical Rating)
ASTM D4870 Standard Test Method for Determination of Total Sediment in Residual Fuels
ASTM D4952 Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test)
ASTM D515 Standard test method for dust abrasion of granular activated carbon
ASTM D5304 Standard Test Method for Assessing Middle Distillate Fuel Storage Stability by Oxygen Overpressure
ASTM D5452 Standard Test Method for Particulate Contamination in Aviation Fuels by Laboratory Filtration*, 2023-05-01 Update
ASTM D6217 Standard Test Method for Particulate Contamination in Middle Distillate Fuels by Laboratory Filtration
ASTM D6227 Standard Specification for Grade 82 Unleaded Aviation Gasoline
ASTM D6426 Standard Test Method for Determining Filterability of Distillate Fuel Oils
ASTM D664 Standard Test Method for Acid Number of Petroleum Products by Potentiometric Titration
ASTM D6751 Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels*, 2023-04-01 Update
ASTM D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water*, 2021-04-01 Update
ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM D7687 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration (Standard + Redline PDF Bundle)*, 2023-04-03 Update
ASTM D7978 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
ASTM D8070 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay*, 2023-07-01 Update
ASTM D888 Standard Test Methods for Dissolved Oxygen in Water
ASTM D910 Standard Specification for Aviation Gasolines
ASTM D974 Standard Test Method for Acid and Base Number by Color-Indicator Titration
ASTM D975 Standard Specification for Diesel Fuel Oils
ASTM E1259 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390176;C
ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM D6469-20 history
2020ASTM D6469-20 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2017ASTM D6469-17 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2014ASTM D6469-14 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2012ASTM D6469-12 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2011ASTM D6469-11 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2008ASTM D6469-08e1 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2008ASTM D6469-08 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
2004ASTM D6469-04 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
1999ASTM D6469-99 Standard Guide for Microbial Contamination in Fuels and Fuel Systems