ASTM E1533-00
Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4'-6-Diamidino-2-2 Phenylindole (DAPI) Staining

Standard No.

ASTM E1533-00

Release Date

2000

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM E1533-00(2006)

Latest

ASTM E1533-00(2006)

Scope

1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining.

1.2 This practice does not cover direct methods for the detection of mycoplasma or other indirect methods such as enzymatical detection or DNA probes.

1.3 This practice does not cover methods for the identification of mycoplasma organisms.

1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E1533-00 Referenced Document

• ASTM E1531 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Growth on Agarose Medium *， 2024-04-21 Update
• ASTM E1532 Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome *， 2024-04-21 Update
• ASTM E1536 Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method *， 2024-04-21 Update

ASTM E1533-00 history

• 2000 ASTM E1533-00(2006) Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4`-6-Diamidino-2-2 Phenylindole (DAPI) Staining
• 2000 ASTM E1533-00 Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4'-6-Diamidino-2-2 Phenylindole (DAPI) Staining
• 1993 ASTM E1533-93 Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4`-6-Diamidino-2-2 Phenylindole (DAPI) Staining