This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives. For further information on the validation, see Clause 8 and Annex B.
EN 14122:2014 Referenced Document
EN ISO 3696:1995 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
EN ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories (ISO/IEC 17025:2005)
EN 14122:2014 history
2014EN 14122:2014 Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography
2003EN 14122:2003 Foodstuffs Determination of vitamin B1 by HPLC Incorporating Corrigendum December 2005