ASTM D7464-14
Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

Standard No.

ASTM D7464-14

Release Date

2014

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM D7464-19

Latest

ASTM D7464-20

Scope

5.1 Representative samples of fuel products and associated substances are required for the determination of microbial contamination in fuels and fuel systems in order to accurately assess the biodeterioration risk posed to the fuel, fuel-system components or both. Uncontrolled microbial contamination can affect fuel specification properties adversely.6 As discussed in Guide D6469, microbes can cause a variety of operational problems, including filter plugging and microbially influenced corrosion (MIC), the latter of which causes valve failure, tank and pipeline failure.

5.2 These practices for microbiological sampling decrease the risk of contaminating samples with extraneous microbes, thereby increasing the probability that the original microbial population in the sample does not change significantly between the time of sampling and the time of testing.

5.3 The objective of sampling for microbiological testing is to obtain representative samples that are likely to reflect the degree and nature of microbial contamination in the system from which the samples are collected. Manual 477 addresses the rational for and design of microbial contamination programs. Recognizing that microbiological contamination is not distributed uniformly throughout fuel systems, both the number and types of samples collected will normally be different from the samples collected per Practice D4057 in order to determine whether product meets specifications.

5.4 The physical, chemical and microbiological property tests to be performed on a sample will dictate the sampling procedures, the sample quantity required, and many of the sample handling requirements.

5.5 Fuel systems are not normally designed to facilitate optimal microbiological sampling. Consequently, the selection of sampling device and sample source reflect compromises between accessibility and suitability for meeting the sample collection objective.

5.6 The guidance provided in Practice D4057 generally applies to this practice as well. Consequently, this practice will address only those procedures that apply uniquely to microbiological sampling.

1.1 This practice covers aspects of sample device preparation and sample handling that prevent samples from becoming contaminated with microorganisms not originally contained within the sample.

1.2 This practice also covers sample handling considerations that reflect the perishability of samples collected for microbiological testing.

1.3 This practice supplements Practice D4057 by providing guidance specific to the manual sampling of fuels when samples are to be tested for microbial contamination.

1.4 The values stated in SI units are to be regarded as s......

ASTM D7464-14 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D1655 Standard Specification for Aviation Turbine Fuels
• ASTM D2069 
• ASTM D2880 Standard Specification for Gas Turbine Fuel Oils
• ASTM D3508 Standard Method for Evaluating Water Testing Membrane Filters for Fecal Coliform Recovery
• ASTM D3699 Standard Specification for Kerosine
• ASTM D396 Standard Specification for Fuel Oils
• ASTM D4057 Standard Practice for Manual Sampling of Petroleum and Petroleum Products
• ASTM D4814 Standard Specification for Automotive Spark-Ignition Engine Fuel
• ASTM D5245 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
• ASTM D6227 Standard Specification for Grade 82 Unleaded Aviation Gasoline
• ASTM D6469 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
• ASTM D6751 Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels
• ASTM D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
• ASTM D910 Standard Specification for Aviation Gasolines
• ASTM D975 Standard Specification for Diesel Fuel Oils

ASTM D7464-14 history

• 2020 ASTM D7464-20 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• 2019 ASTM D7464-19 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• 2014 ASTM D7464-14 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• 2008 ASTM D7464-08(2013) Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
• 2008 ASTM D7464-08 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing