ISO 29200:2013 PDF

Scope: The purpose of this International Standard is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters,etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments.Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters,effluents, etc.

ISO 29200:2013 Referenced Document

EN 14735 Characterization of waste - Preparation of waste samples for ecotoxicity tests
ISO 10381-6 Soil quality - Sampling - Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390 Soil, treated biowaste and sludge - Determination of pH*， 2021-03-31 Update
ISO 10694 Soil quality - Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260 Soil quality - Determination of effective cation exchange capacity and base saturation level using barium chloride solution*， 2018-05-01 Update
ISO 11269-2:2012 Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 11465 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method; technical corrigendum 1
ISO/TS 21268-1 Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials - Part 1: Batch test using a liquid to solid ratio of 2 l/kg dry matter
ISO/TS 21268-2 Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter

2013 ISO 29200:2013 Soil quality.Assessment of genotoxic effects on higher plants.Vicia faba micronucleus test