ISO 11268-2:2012 PDF

Scope: This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil). Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry’s constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,0133 Pa at 25 °C. This method does not take into account the persistence of the substance during the test.

ISO 11268-2:2012 Referenced Document

ISO 10381-6 Soil quality - Sampling - Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 10390 Soil, treated biowaste and sludge - Determination of pH*， 2021-03-31 Update
ISO 10694 Soil quality - Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260 Soil quality - Determination of effective cation exchange capacity and base saturation level using barium chloride solution*， 2018-05-01 Update
ISO 11268-1 Soil quality - Effects of pollutants on earthworms - Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andrei
ISO 11277 Soil quality*， 2024-03-01 Update
ISO 11465 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method; technical corrigendum 1

2023 ISO 11268-2:2023 Soil quality — Effects of pollutants on earthworms — Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species
2012 ISO 11268-2:2012 Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei
1998 ISO 11268-2:1998 Soil quality - Effects of pollutants on earthworms (Eisenia fetida) - Part 2: Determination of effects on reproduction