ISO 11268-2:2023 PDF

Scope: This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei. Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.

ISO 11268-2:2023 Referenced Document

ISO 10390 Soil, treated biowaste and sludge - Determination of pH
ISO 10694 Soil quality - Determination of organic and total carbon after dry combustion (elementary analysis)
ISO 11260 Soil quality - Determination of effective cation exchange capacity and base saturation level using barium chloride solution
ISO 11277 Soil quality*， 2024-03-01 Update
ISO 11465 Soil quality; determination of dry matter and water content on a mass basis; gravimetric method; technical corrigendum 1

2023 ISO 11268-2:2023 Soil quality — Effects of pollutants on earthworms — Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species
2012 ISO 11268-2:2012 Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei
1998 ISO 11268-2:1998 Soil quality - Effects of pollutants on earthworms (Eisenia fetida) - Part 2: Determination of effects on reproduction