This International Standard specifies a method for the determination of the contents of free a-, p-, y-, and 5-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.
NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.
ISO 9936:2006 Referenced Document
ISO 661 Animal and vegetable fats and oils - Preparation of test sample
ISO 9936:2006 history
2016ISO 9936:2016 Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography
2011ISO 9936:2006/Amd 1:2011 Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography - Amendment 1: Updating of reagents and confirmation of statistical data validity
2008ISO 9936:2006/Cor 1:2008 Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography; Technical Corrigendum 1
2006ISO 9936:2006 Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography
1997ISO 9936:1997 Animal and vegetable fats and oils - Determination of tocopherols and tocotrienols contents - Method using high-performance liquid chromatography