ASTM E645-13
Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems

Standard No.

ASTM E645-13

Release Date

2013

Published By

American Society for Testing and Materials (ASTM)

Status

Be replaced

Replace By

ASTM E645-18

Latest

ASTM E645-18

Scope

5.1 This practice determines potentially effective microbicides for use in cooling water systems using cooling water and deposits/biofilm obtained from the field. The addition of deposits/biofilms addresses the need to include the major source of microorganisms in cooling water systems. Even with this addition, laboratory results may not be totally predictive of microbicidal effectiveness in the field. This is because conditions in the field affecting microbicide effectiveness are difficult to mimic in the laboratory. These conditions that affect microbicide efficacy include blow-down rate, addition of makeup water, water hardness, hydrocarbon leaks, pH, sediment loading, dissolved solids, microbes in slime (biofilms), and deposits (salts, iron minerals, organics, and so forth) on surfaces. An additional factor is the difficulty in enumerating all microbes in the water due to the lack of adequate recovery media. Guidelines that address formation of and testing for surface-attached microbes (biofilms) may be found in Guide E1427, while a guideline for unconventional measurement of microbes is found in Guide E1326.

1.1 This practice outlines a procedure for evaluating the efficacy of microbicides (algicides, bactericides, and fungicides) that will be used for controlling microbial growth in cooling water systems. The microbicides will be evaluated using simulated or real cooling tower water against (1) microbes from cooling water, (2) microbes in microbiological deposits (biofilms) from operating cooling systems, or (3) microorganisms known to contaminate cooling water systems, or a combination thereof. This practice should be performed by individuals familiar with microbiological techniques.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E645-13 Referenced Document

• ASTM D3731 Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters
• ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
• ASTM D4412 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
• ASTM E1054 Standard Practices for Evaluation of Inactivators of Antimicrobial Agents*， 2022-10-01 Update
• ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
• ASTM E1427 Standard Guide for Selecting Test Methods to Determine the Effectiveness of Antimicrobial Agents and Other Chemicals for the Prevention, Inactivation and Removal of Biofilm *， 2024-04-22 Update
• ASTM E2756 Standard Terminology Relating to Antimicrobial and Antiviral Agents*， 2019-11-01 Update

ASTM E645-13 history

• 2018 ASTM E645-18 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
• 2013 ASTM E645-13 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
• 2007 ASTM E645-07 Standard Test Method for Efficacy of Microbicides Used in Cooling Water Systems
• 2002 ASTM E645-02a Standard Test Method for Efficacy of Microbicides Used in Cooling Systems
• 2002 ASTM E645-02 Standard Test Method for Efficacy of Microbicides Used in Cooling Systems
• 1997 ASTM E645-97 Standard Test Method for Efficacy of Microbicides Used in Cooling Systems