1.1 This practice outlines a procedure for evaluating the efficacy of microbicides (algicides, bactericides, and fungicides) that will be used for controlling microbial growth in cooling water systems. The microbicides will be evaluated using simulated or real cooling tower water against (1) microbes from cooling water, (2) microbes in microbiological deposits (biofilms) from operating cooling systems, or (3) microorganisms known to contaminate cooling water systems, or a combination thereof. This practice should be performed by individuals familiar with microbiological techniques.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E645-18 Referenced Document
ASTM D3731 Standard Practices for Measurement of Chlorophyll Content of Algae in Surface Waters
ASTM D4012 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D4412 Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits
ASTM E1054 Standard Practices for Evaluation of Inactivators of Antimicrobial Agents*, 2022-10-01 Update
ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM E1427 Standard Guide for Selecting Test Methods to Determine the Effectiveness of Antimicrobial Agents and Other Chemicals for the Prevention, Inactivation and Removal of Biofilm *, 2024-04-21 Update
ASTM E2756 Standard Terminology Relating to Antimicrobial and Antiviral Agents*, 2019-11-01 Update
ASTM E645-18 history
2018ASTM E645-18 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
2013ASTM E645-13 Standard Practice for Evaluation of Microbicides Used in Cooling Water Systems
2007ASTM E645-07 Standard Test Method for Efficacy of Microbicides Used in Cooling Water Systems
2002ASTM E645-02a Standard Test Method for Efficacy of Microbicides Used in Cooling Systems
2002ASTM E645-02 Standard Test Method for Efficacy of Microbicides Used in Cooling Systems
1997ASTM E645-97 Standard Test Method for Efficacy of Microbicides Used in Cooling Systems