1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751, and D7467), system deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E1259-18 Referenced Document
ASTM D1655 Standard Specification for Aviation Turbine Fuels
ASTM D4814 Standard Specification for Automotive Spark-Ignition Engine Fuel
ASTM D5465 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D6227 Standard Specification for Grade 82 Unleaded Aviation Gasoline
ASTM D6293 Standard Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic (O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography
ASTM D6469 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D6729 Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography*, 2020-06-01 Update
ASTM D6733 Standard Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography*, 2020-11-01 Update
ASTM D6751 Standard Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels*, 2023-04-01 Update
ASTM D6974 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures*, 2020-05-01 Update
ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water*, 2021-04-01 Update
ASTM D7464 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing*, 2020-05-01 Update
ASTM D7467 Standard Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)*, 2023-10-01 Update
ASTM D7687 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration (Standard + Redline PDF Bundle)*, 2023-04-03 Update
ASTM D7978 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method*, 2019-07-01 Update
ASTM D910 Standard Specification for Aviation Gasolines
ASTM D975 Standard Specification for Diesel Fuel Oils
ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
ASTM E1259-18 history
2023ASTM E1259-23 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
2018ASTM E1259-18 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
2016ASTM E1259-16 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390&xb0;C
2010ASTM E1259-10 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390&176;C
2005ASTM E1259-05 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390176;C
2001ASTM E1259-01 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390176;C
1994ASTM E1259-94 Standard Test Method for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390176;C