ISO 6888-2:2021
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium

Standard No.

ISO 6888-2:2021

Release Date

2021

Published By

International Organization for Standardization (ISO)

Status

Be replaced

Replace By

ISO 6888-2:2021/Amd 1:2023

Latest

ISO 6888-2:2021/Amd 1:2023

Scope

This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]). This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium; — background flora that can obscure the colonies being sought. Nevertheless, both ISO 6888-1 and this document are given equivalent status.  1 

ISO 6888-2:2021 Referenced Document

• ISO 11133 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media AMENDMENT 1
• ISO 7218 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations AMENDMENT 1

ISO 6888-2:2021 history

• 2023 ISO 6888-2:2021/Amd 1:2023 Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Method using rabbit plasma fibrinogen agar medium — Amendment 1
• 2021 ISO 6888-2:2021 Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium
• 2003 ISO 6888-2:1999/Amd 1:2003 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Technique using rabbit plasma fibrinogen agar medium; Amendment 1: Inclusion of
• 1999 ISO 6888-2:1999 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Technique using rabbit plasma fibrinogen agar medium