ASTM F2997-21
Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis

Standard No.

ASTM F2997-21

Release Date

2021

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM F2997-21

Scope

1.1?This practice defines a method for the estimation of calcium content at multiple time points in living cell cultures that have been cultured under conditions known to promote mineralization. The practice involves applying a fluorescent calcium-chelating dye that binds to the

ASTM F2997-21 Referenced Document

• ASTM F2312 Standard Terminology Relating to Tissue Engineered Medical Products
• ASTM F3294 Standard Guide for Performing Quantitative Fluorescence Intensity Measurements in Cell-based Assays with Widefield Epifluorescence Microscopy

ASTM F2997-21 history

• 2021 ASTM F2997-21 Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis
• 2013 ASTM F2997-13 Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis