ISO 20635:2018 PDF

Scope: This document specifies a method for the determination of vitamin C (L-ascorbic acid) present in all forms of infant and adult formulas (powders, ready-to-feed liquids and liquid concentrates), using (ultra) high performance liquid chromatography with ultraviolet detection (U)HPLC-UV. The application range runs from 2,5 mg/100 g (limit of quantification) to 50 mg/100 g expressed in the product as consumed. The method is able to distinguish between D-ascorbic acid (isoascorbic- or erythorbic acid) and L-ascorbic acid.

ISO 20635:2018 Referenced Document

CODEX STAN 72-2007 STANDARD FOR INFANT FORMULA AND FORMULAS FOR SPECIAL MEDICAL PURPOSES INTENDED FOR INFANTS
ISO 1042:1998 Laboratory glassware - One-mark volumetric flasks
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

2018 ISO 20635:2018 Infant formula and adult nutritionals - Determination of vitamin C by (ultra) high performance liquid chromatography with ultraviolet detection ((U)HPLC-UV)