ISO 16649-1:2018
Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide

Standard No.

ISO 16649-1:2018

Release Date

2018

Published By

International Organization for Standardization (ISO)

Latest

ISO 16649-1:2018

Scope

This document specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9] [10][13][14][17][18][19][20]. It is applicable to — products intended for human consumption, — products intended for feeding animals, — environmental samples in the area of food production and food handling, and — samples from the primary production stage such as animal faeces, dust, and swabs. WARNING — Some strains of Escherichia coli may grow poorly or not at all in media incubated at 44 °C. This includes strains of E. coli O157:H7 and O157:H-. Additionally, some strains of Escherichia coli, notably those belonging to serotype O157:H7, are mostly β-glucuronidase negative[11]. Consequently, some strains of E. coli, including pathogenic ones, will not be detected by this method. β-glucuronidase activity may also be exhibited at 44 °C by certain other members of the Enterobacteriaceae, notably Shigella[15] and Salmonella[16].

ISO 16649-1:2018 Referenced Document

• ISO 11133:2014 Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
• ISO 13307:2013 Microbiology of food and animal feed - Primary production stage - Sampling techniques
• ISO 16649-2:2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of -glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 °C using 5-bromo-4-chloro-3-indolyl -D-glucoronide
• ISO 16649-3:2015 Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
• ISO 17468:2016 Microbiology of the food chain - Technical requirements and guidance on establishment or revision of a standardized reference method
• ISO 17604:2015 Microbiology of the food chain - Carcass sampling for microbiological analysis
• ISO 18593:2004 Microbiology of food and animal feeding stuffs - Horizontal methods for sampling techniques from surfaces using contact plates and swabs
• ISO 6887-1:2017 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions
• ISO 6887-2:2017 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products
• ISO 6887-3:2017 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 3: Specific rules for the preparation of fish and fishery products
• ISO 6887-4:2017 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 4: Specific rules for the preparation of miscellaneous products
• ISO 6887-5:2010 Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 5: Specific rules for the preparation of milk and milk products
• ISO 6887-6:2013 Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage
• ISO 707:2008 Milk and milk products - Guidance on sampling
• ISO 7218:2007 Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
• ISO/TS 17728:2015 Microbiology of the food chain - Sampling techniques for microbiological analysis of food and feed samples

ISO 16649-1:2018 history

• 2018 ISO 16649-1:2018 Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide
• 2001 ISO 16649-1:2001 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of -glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3-indolyl -D-glucuronide