DIN EN ISO 21149:2017 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017); German version EN ISO 21149:2017
This document gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics - by counting the colonies on agar medium after aerobic incubation, or - by checking the absence of bacterial growth after enrichment. Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e. g. certain water immiscible products). Other methods (e. g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable. If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
DIN EN ISO 21149:2017 Referenced Document
DIN EN ISO 18415:2017 Cosmetics - Microbiology - Detection of specified and non-specified microorganisms (ISO 18415:2017); German version EN ISO 18415:2017
DIN EN ISO 21148:2017 Cosmetics - Microbiology - General instructions for microbiological examination (ISO 21148:2017); German version EN ISO 21148:2017
DIN EN ISO 22717:2016 Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa
DIN EN ISO 22718:2016 Cosmetic products – Microbiology – Detection of Staphylococcus aureus
DIN EN ISO 29621:2017 Cosmetics - Microbiology - Guidelines for the risk assessment and identification of microbiologically low-risk products (ISO 29621:2017); German version EN ISO 29621:2017
EN 1040:2005 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1)*, 2024-04-20 Update
EN 12353:2013 Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
ISO 18415:2007 Cosmetics - Microbiology - Detection of specified and non-specified microorganisms
ISO 18416:2015 Cosmetics - Microbiology - Detection of Candida albicans
ISO 21148:2017 Cosmetics - Microbiology - General instructions for microbiological examination
ISO 21150:2015 Cosmetics - Microbiology - Detection of Escherichia coli
ISO 22717:2015 Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa
ISO 22718:2015 Cosmetics — Microbiology — Detection of Staphylococcus aureus
ISO 29621:2017 Cosmetics - Microbiology - Guidelines for the risk assessment and identification of microbiologically low-risk products
DIN EN ISO 21149:2017 history
2023DIN EN ISO 21149:2023-01 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017 + Amd 1:2022); German version EN ISO 21149:2017 + A1:2022
2017DIN EN ISO 21149:2017 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017); German version EN ISO 21149:2017
2009DIN EN ISO 21149:2009 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2006); English version of DIN EN ISO 21149:2009-10