This International Standard provides general guidance for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. The microorganisms specified in this International Standard may vary from country to country in accordance with national practices or regulations. In order to ensure the quality of the product and safety for consumers, it is advisable to carry out an appropriate microbiological risk analysis to determine the types of cosmetic products to which this International Standard is applicable. Products that pose a low microbiological risk (see ISO 29621) include those with low water activity, alcoholic-aqueous products, products with extreme pH values, etc. The method described in this International Standard is not based on the detection of Escherichia coli in a -selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Depending on the detection limit required, other methods may be appropriate. NOTE For the detection of Escherichia coli, subculturing on non-selective culture media can be carried out, followed by appropriate identification steps (e.g. using identification kits). Due to the wide variety of cosmetic products within this scope, this process may not be suitable in all details for some products (e.g. certain water-immiscible products). Other International Standards (ISO 18415) may be appropriate. Alternatively, other methods (e.g. automated) can be used for the examinations listed here, provided that their equivalence has been proven or the method has otherwise proven to be suitable.
EN ISO 21150:2015 Referenced Document
EN 1040:2005 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1)*， 2024-04-20 Update
EN 12353:2013 Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
ISO 18415:2007 Cosmetics - Microbiology - Detection of specified and non-specified microorganisms
ISO 21148:2005 Cosmetics - Microbiology - General instructions for microbiological examination
ISO 21149:2006 Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria
ISO 29621:2010 Cosmetics - Microbiology - Guidelines for the risk assessment and identification of microbiologically low-risk products
EN ISO 21150:2015 history
2022EN ISO 21150:2015/A1:2022 Cosmetics - Microbiology - Detection of Escherichia coli - Amendment 1 (ISO 21150:2015/Amd 1:2022)
2015EN ISO 21150:2015 Cosmetics - Microbiology - Detection of Escherichia coli
2009EN ISO 21150:2009 Cosmetics - Microbiology - Detection of Escherichia coli