Canine Hepatitis Vaccine and Canine Adenovirus Type 2 Vaccine shall be prepared from virus-bearing cell culture fluids. Only Master Seed Virus which has been established as pure, safe, and immunogenic shall be used in preparing the production seed virus for vaccine production. All serials shall be prepared from the first through the fifth passage from the Master Seed Virus.
CFR 9-113.305-2014 history
2014CFR 9-113.305-2014 Animals and animal products. Part113:Standard requirements. Section113.305:Canine Hepatitis and Canine Adenovirus Type 2 Vaccine.