PD CEN/TS 16707:2014
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Polymerase chain reaction (PCR) based screening strategies

Standard No.

PD CEN/TS 16707:2014

Release Date

2014

Published By

European Committee for Standardization (CEN)

Latest

PD CEN/TS 16707:2014

Replace

FprCEN/TS 16707-2014

Scope

This Technical Specification describes screening strategies for the detection of genetically modified (GM) DNA in food products by means of PCR methods. The strategies have been established for food matrices, but it can also be applied to other matrices (e. g. feed, seed and samples from field grown plants). Detection of GM DNA is based on PCR methods targeting segments of transgenic DNA sequences (genetic elements, genetic constructs or insertion sites of transgenes). Various combinations of these PCR methods are involved in screening strategies. The methods are applied simultaneously or hierarchically. The general strategy is based on the matrix approach. Examples for the implementation and application of this approach are described. In order to ensure reliable analytical results, the document also provides guidelines for the validation of the performance of qualitative PCR methods applied in screening approaches.

PD CEN/TS 16707:2014 Referenced Document

• EN ISO 21569:2005 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods (ISO 21569:2005)
• EN ISO 21570:2005 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods (ISO 21570:2005)*， 2024-04-20 Update
• EN ISO 21571:2005 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Nucleic acid extraction (ISO 21571:2005)
• EN ISO 24276:2006 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - General requirements and definitions (ISO 24276:2006)
• ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definitions
• ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
• ISO 5725-3:1994 Accuracy (trueness and precision) of measurement methods and results - Part 3: Intermediate measures of the precision of a standard measurement method
• ISO 5725-4:1994 Accuracy (trueness and precision) of measurement methods and results - Part 4: Basic methods for the determination of the trueness of a standard measurement method
• ISO 5725-5:1998 Accuracy (trueness and precision) of measurement methods and results - Part 5: Alternative methods for the determination of the precision of a standard measurement method
• ISO 5725-6:1994 Accuracy (trueness and precision) of measurement methods and results - Part 6: Use in practice of accuracy values

PD CEN/TS 16707:2014 history

• 2014 PD CEN/TS 16707:2014 Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Polymerase chain reaction (PCR) based screening strategies