This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The - glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166, determines the total vitamin B6 by microbiological assay.
EN 14164:2014 Referenced Document
EN 14166:2009 Foodstuffs - Determination of vitamin B6 by microbiological assay
EN 14663:2005 Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC*， 2024-04-20 Update
EN ISO 3696:1995 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
EN ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories (ISO/IEC 17025:2005)
ISO 5725:1986 Precision of test methods; Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
EN 14164:2014 history
2014EN 14164:2014 Foodstuffs - Determination of vitamin B6 by high performance chromatography
2008EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC