CEN ISO/TS 17919:2013 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
This Technical Specification specifies a horizontal method for the detection of Clostridia carrying genes for the botulinum neurotoxins A, B, E and F at the molecular level using a PCR method. This procedure detects the genes and not the toxins, so a positive result does not necessarily mean that these toxins are present in the sample examined. This Technical Specification is applicable to products for human consumption, feed and environmental samples. The PCR methods for detecting gene sequences encoding specific types of toxins are described in Appendices B and C.
CEN ISO/TS 17919:2013 Referenced Document
ISO 20837:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection
ISO 20838:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods
ISO 22174:2005 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions
ISO 6887-1:1999 Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations
CEN ISO/TS 17919:2013 history
2013CEN ISO/TS 17919:2013 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia