NB/SH/T 0860-2013 (English Version) PDF

Scope: This standard specifies the method for detecting and counting heterotrophic bacteria and fungi in liquid fuels with a kinematic vis osity not greater than 24 mm/s under normal temperature conditions using membrane filtration. This standard applies to liquid fuels. This standard can be performed in the field or in the laboratory. The ability of individual microorganis s to form colonies on a specific culture medium depends on the taxonomic and ecological status of the enumerated species, the chemical properties of the culture medium, and the culture conditions. Therefore, test results should not be interpreted as absolute values but should be used as part of diagnostics and environmental monitoring along with other parameters in accordance with ASTM D6469. This standard provides multiple options for transferring microorganis s in fuel samples to the membrane, such as filtration method, filtration volume, dilution filtration, culture medium used for microorganis s, and culture temperature. The flexibility of the test simplifies the diagnostic process. When this method is used for condition testing, the same procedure should always be chosen.

NB/SH/T 0860-2013 Referenced Document

ASTM D3508 Standard Method for Evaluating Water Testing Membrane Filters for Fecal Coliform Recovery
ASTM D5245 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
ASTM D5259 Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
ASTM D6469 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D7463 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water*， 2021-04-01 Update
ASTM D7464-08 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing*， 2024-04-20 Update
ASTM E1326 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
GB/T 4756 Method for manual sampling of petroleum liquids*， 2015-12-31 Update
GB/T 6682-2008 Water for analytical laboratory use.Specification and test methods

2013 NB/SH/T 0860-2013 Standard practice for enumeration of viable bacteria and fungi in liquid fuels.Filtration and culture procedures