VDI 4253 Blatt 4-2013
Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI
- Standard No.
- VDI 4253 Blatt 4-2013
- Release Date
- 2013
- Published By
- Association of German Mechanical Engineers
- Latest
- VDI 4253 Blatt 4-2013
- Scope
- The guideline describes an evaluation method for quantification of the total cell count of microorganisms by microscopic counting after staining of DNA-containing structures. It is a cultivation-independent method for analysis of ambient air samples, emission samples and indoor air samples in the course of which living and dead microorganisms are detected. The use of this analytical method is particularly advisable if microorganisms are subject to hostile environmental impacts on their way from the emission source to the sampling location. This guideline is aimed at a concentration range starting from 105 GZZ/m3 for sampling with impingers at an air sampling volume of 0,5 m3. For lower expected total cell counts higher air sampling volumes are necessary which can be obtained by means of filtration methods with polycarbonate filters.
VDI 4253 Blatt 4-2013 history
- 2013 VDI 4253 Blatt 4-2013 Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI
- 2011 VDI 4253 Blatt 4-2011 Erfassen luftgetragener Mikroorganismen und Viren in der Aussenluft - Bestimmung der Gesamtzellzahl mittels Fluoreszenzanalyse nach Anfaerbung mit DAPI
