BS DD CEN/TS 16115-1:2011 Ambient air quality. Measurement of bioaerosols. Determination of moulds using filter sampling systems and culture-based analyses
This Technical Specification describes the measurement of moulds in ambient air in order to identify, quantify
and characterize bioaerosol pollution in ambient air resulting from emissions from different sources.
The method described specifies the sampling of moulds as part of the suspended particulate matter (SPM,
here particles with aerodynamic diameter up to ca. 30 μm) using a filter sampling system with gelatine/polycarbonate
filter combination followed by the culture-based analyses on DG18 agar. The sampling duration can
be varied between 10 min to 24 h. The health effect of bioaerosols is not limited to any particle fraction,
therefore, this document describes the sampling of moulds as part of the suspended particulate matter as a
convention method.
NOTE The sampling method described in this document in principle is likely to be appropriate for the sampling of
actinomycetes and other spore-forming bacteria (resistant to desiccation). For these species a special analytical
procedure using different culture media should be applied, but this is not within the scope of this document.
The standard method set out in this Technical Specification is accepted by convention as reference method.
The measured quantity, here the number of colony forming units per cubic meter (CFU/m), is determined by
the inlet design of the sampling head, the associated operational parameters and the analytical procedure.
Standardized methods for sampling, detection and enumeration of moulds including standards for sampling
strategies are important for comparative assessment of moulds in ambient air. Before doing any
measurements a plan for the measurement strategy is necessary (see CEN/TS 16115-2 [5]).
WARNING — The use of this Technical Specification may involve hazardous materials, operations and
equipment. This Technical Specification does not purport to address all the safety problems
associated with its use. It is the responsibility of the user of this standard to establish appropriate
safety and health practices and determine the applicability of regulatory limitations prior to use.
BS DD CEN/TS 16115-1:2011 Referenced Document
EN ISO 8199:2007 Water quality - General guidance on the enumeration of micro-organisms by culture (ISO 8199:2005)*， 2024-04-19 Update
BS DD CEN/TS 16115-1:2011 history
2011BS DD CEN/TS 16115-1:2011 Ambient air quality. Measurement of bioaerosols. Determination of moulds using filter sampling systems and culture-based analyses