ISO 22030:2005 PDF

Scope: This International Standard describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (Brassica rapa CrGC syn. Rbr) and oat (Avena sativa). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants. By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test can be used to assess soil quality, especially the function of the soil as a habitat for plants. Annex A describes modifications allowing use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals. This method is not applicable to volatile substances, i.e. substances for which H (Henry’s constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

ISO 22030:2005 Referenced Document

ISO 11268-1:1993 Soil quality; effects of pollutants on earthworms (Eisenia fetida); part 1: determination of acute toxicity using artificial soil substrate
ISO 11268-2:1998 Soil quality - Effects of pollutants on earthworms (Eisenia fetida) - Part 2: Determination of effects on reproduction
ISO 11269-2 Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants*， 2012-01-01 Update
ISO 15176:2002 Soil quality - Characterization of excavated soil and other soil materials intended for re-use
ISO 15799 Soil quality — Guidance on the ecotoxicological characterization of soils and soil materials*， 2019-03-12 Update

2005 ISO 22030:2005 Soil quality - Biological methods - Chronic toxicity in higher plants