DIN EN 15607:2009
Foodstuffs - Determination of d-biotin by HPLC; English version of DIN EN 15607:2009-09
- Standard No.
- DIN EN 15607:2009
- Release Date
- 2009
- Published By
- German Institute for Standardization
- Status
- Replace By
- DIN EN 15607:2009-09
- Latest
- DIN EN 15607:2009-09
- Replace
- DIN EN 15607:2007
- Scope
- This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 pg/100 g to 200 pg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.
DIN EN 15607:2009 Referenced Document
- EN ISO 3696:1995 Water for Analytical Laboratory Use- Specification and Test Methods (ISO 3696 : 1987)
DIN EN 15607:2009 history
- 2009 DIN EN 15607:2009-09 Foodstuffs - Determination of d-biotin by HPLC; German version EN 15607:2009
- 2009 DIN EN 15607:2009 Foodstuffs - Determination of d-biotin by HPLC; English version of DIN EN 15607:2009-09
- 0000 DIN EN 15607:2007
