BS EN 12393-2:2008 Foods of plant origin - Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and cleanup
This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for
quantitative determination of pesticide residues.
Different solvents can be used for this purpose. These pesticide residues are generally associated with other
co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed,
several techniques can be used.
This standard contains the following extraction and clean-up methods that have been subjected to
interlaboratory studies and /or are adopted throughout Europe:
? method L: Extraction with acetone, liquid-liquid partition with dichloromethane and clean-up on a silica
gel/charcoal column [1];
? method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if
necessary clean-up on Florisil? 1) [2], [3], [4];
? method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl
acetate and clean-up with gel permeation and silica gel chromatography [5], [6];
? method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography
[7].
This European Standard specifies the details of methods L to P for the extraction and the clean-up of food
samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up
are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation
chromatography.
A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list
of indicative applicability of the method to different pesticides are given for each method, wherever possible.
BS EN 12393-2:2008 history
2013BS EN 12393-2:2013 Foods of plant origin. Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS. Methods for extraction and clean-up
2008BS EN 12393-2:2008 Foods of plant origin - Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and cleanup