EN 15452:2008 Fertilizers - Determination of chelating agents - Determination of iron chelated by o,p-EDDHA by reversed phase HPLC [Superseded: CEN CEN/TS 15452]
This European Standard specifies a method for the chromatographic determination of the amount of iron
chelated by each of the individual isomers of the chelating agent ortho-para EDDHA (o,p-EDDHA) in fertilizers.
The method allows the identification of this chelating agent and the determination of the water soluble fraction
of iron chelated by this chelating agent. The method is not applicable for the determination of the amount of
free chelating agent.
NOTE 1 This method has been shown to be also suitable for the determination of the amount of iron chelated by each
of the individual isomers of the chelating agent ortho-ortho EDDHA (o,o-EDDHA) in fertilizers.
NOTE 2 o,o-EDDHA and o,p-EDDHA are abbreviations used in this European Standard for the sake of simplicity. For
complete names see Annex C.
NOTE 3 The substances o,o-EDDHA and o,p-EDDHA both exist as different stereoisomers. For o,o-EDDHA a meso
form and a d/l pair (the racemic isomers) exist, for o,p-EDDHA two different d/l pairs exist. All four stereoisomers are
observed separately in this method.
NOTE 4 Currently, an analytically pure standard only exists for o,o-EDDHA. The method for o,p-EDDHA has been
developed with an o,p-EDDHA standard containing an uncertain concentration of o,p-EDDHA.
EN 15452:2008 Referenced Document
EN 1482-2 Fertilizers and liming materials - Sampling and sample preparation - Part 2: Sample preparation
EN 15452:2008 history
2008EN 15452:2008 Fertilizers - Determination of chelating agents - Determination of iron chelated by o,p-EDDHA by reversed phase HPLC [Superseded: CEN CEN/TS 15452]