EN ISO 22174:2005 PDF

Scope: This International Standard describes general requirements for the in vitro reproduction (amplification) of nucleic acid sequences (DNA or RNA). It applies to the examination of foods and isolates obtained from them for microbial contamination using the polymerase chain reaction (PCR). The minimum requirements set out in this International Standard are intended to ensure comparable and reproducible results in different laboratories. This International Standard has been prepared for the detection of pathogenic microorganisms in or isolated from food and feed matrices. However, it can also be applied to other matrices, e.g. B. Environmental samples, and can be used to detect non-pathogenic microorganisms.

EN ISO 22174:2005 Referenced Document

ISO 20837:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection*， 2006-04-01 Update
ISO 20838:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods*， 2006-04-01 Update
ISO 3534-1:1993 Statistics; vocabulary and symbols; part 1: probability and general statistical terms
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definitions
ISO/IEC 17025:1999 General requirements for the competence of testing and calibration laboratories

2005 EN ISO 22174:2005 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions