This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high
performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine,
pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-
glycosylated forms are not taken into account. These can be determined with the method given in EN 14663
[1] by which the different vitamers of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and
individually quantified. A third European Standard (EN 14166 1 ) [2] determines the total vitamin B6 by
microbiological assay.
BS EN 14164:2008 history
2014BS EN 14164:2014 Foodstuffs. Determination of vitamin B6 by high performance chromatography
2008BS EN 14164:2008 Foodstuffs - Determination of vitamin B6 by HPLC