ASTM D5916-96(2002)
Standard Test Method for Detection and Enumeration of Clostridium perfringens from Water and Extracted Sediments by Membrane Filtration (MF)

Standard No.

ASTM D5916-96(2002)

Release Date

1996

Published By

American Society for Testing and Materials (ASTM)

Status

Withdraw 2011-01

Latest

ASTM D5916-96(2002)

Scope

Clostridium perfringens is a strict obligate anaerobe that is found in fecal material. Under moderately adverse conditions these organisms produce endospores that can withstand extreme environmental conditions and are conservative tracers of past and present pollution in fresh and marine waters and sediments.

1.1 This test method can enumerate Clostridium perfringens spores and vegetative cells from marine water, sediment, wastewater, ambient water, and drinking water. Since C. perfringens spores are present in large numbers in human and animal wastes and are resistant to wastewater treatment practices, extremes in temperature, and environmental stress, they are an indicator of present fecal contamination as well as a conservative tracer of past fecal contamination. It is the user''s responsibility to ensure the validity of this test method for waters of untested matrices.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D5916-96(2002) Referenced Document

• ASTM D1129 Standard Terminology Relating to Water*， 1999-04-19 Update
• ASTM D1193 Standard Specification for Reagent Water*， 1999-04-19 Update
• ASTM D1888 Methods of Test for Particulate and Dissolved Matter in Water
• ASTM D2777 Standard Practice for Determination of Precision and Bias of Applicable Methods of Committee D-19 on Water*， 1998-04-19 Update
• ASTM D3370 Standard Practices for Sampling Water from Closed Conduits
• ASTM D3863 Standard Test Method for Retention Characteristics of 0.40 to 0.45-181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality
• ASTM D3870 Standard Practice for Establishing Performance Characteristics for Colony Counting Methods in Microbiology
• ASTM D5465 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
• ASTM E200 Standard Practice for Preparation, Standardization, and Storage of Standard and Reagent Solutions for Chemical Analysis*， 1997-04-19 Update

ASTM D5916-96(2002) history

• 1996 ASTM D5916-96(2002) Standard Test Method for Detection and Enumeration of Clostridium perfringens from Water and Extracted Sediments by Membrane Filtration (MF)
• 1996 ASTM D5916-96 Standard Test Method for Detection and Enumeration of Clostridium perfringens from Water and Extracted Sediments by Membrane Filtration (MF)