ISO 11866-1:2005 Milk and milk products - Enumeration of presumptive Escherichia coli - Part 1: Most probable number technique using 4-methylumbelliferyl--D-glucuronide (MUG)
International Organization for Standardization (ISO)
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ISO 11866-1:2005
Scope
This part of ISO 11866⎪IDF 170 specifies a combined method for the enumeration of presumptive Escherichia
coli and of presumptive coliforms by means of a culture technique involving a liquid medium with MUG, and
calculation of the number of presumptive Escherichia coli and/or coliforms per gram or per millilitre by the
most probable number (MPN) technique after incubation at 30 °C.
It is a more rapid method than that described in ISO 7251 as the incubation time is reduced (omission of
several enrichment steps).
The method is applicable to
-- milk, liquid milk products,
-- dried milk, dried sweet whey, dried buttermilk, lactose,
-- acid casein, lactic casein and rennet casein,
-- caseinate and dried acid whey,
-- cheese and processed cheese,
-- butter,
-- frozen milk products (including edible ices), and
-- custard, desserts and cream.
The method specified in this part of ISO 11866⎪IDF 170 is preferred for samples in which comparatively low
numbers of presumptive Escherichia coli and/or other presumptive coliforms (less than 100 per gram or 10 per
millilitre) are suspected.
ISO 11866-1:2005 Referenced Document
ISO 7218 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations AMENDMENT 1*, 2007-08-15 Update
ISO 11866-1:2005 history
2005ISO 11866-1:2005 Milk and milk products - Enumeration of presumptive Escherichia coli - Part 1: Most probable number technique using 4-methylumbelliferyl--D-glucuronide (MUG)
1997ISO 11866-1:1997 Milk and milk products - Enumeration of presumptive Escherichia coli - Part 1: Most probable number technique