ISO 16221:2001 PDF

Scope: This International Standard specifies five methods for determining the ultimate aerobic biodegradability of organic compounds in the marine environment by aerobic microorganisms in static aqueous test systems. Standard degradation methods developed for testing in fresh water are modified and adapted to marine conditions. These methods are the DOC die-away test (ISO 7827), the closed bottle test (ISO 10707), the two-phase closed bottle test (ISO 10708), the CO2 evolution test (ISO 9439) and the CO2 headspace test (ISO 14593). The methods apply to organic compounds which a) are water-soluble under the conditions of the test used; b) are poorly water-soluble under the conditions of the test used, in which case special measures may be necessary to achieve good dispersion of the compound (see for example, ISO 10634); c) are volatile, provided that an appropriate test with suitable conditions is used; d) are not inhibitory to the test microorganisms at the concentration chosen for the tests. The presence of inhibitory effects can be determined as specified in this International Standard. NOTE The conditions described in this International Standard do not always correspond to the optimal conditions for allowing the maximum degree of biodegradation to occur. For biodegradation methods in fresh water see ISO 14593 and ISO 15462, and for biodegradation at low concentrations see ISO 14592.

ISO 16221:2001 Referenced Document

ISO 10707 Water quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of biochemical oxygen demand (closed bottle test)
ISO 10708 Water quality - Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds - Determination of biochemical oxygen demand in a two-phase closed bottle test
ISO 14592-1 Water quality -Evaluation of the aerobic biodegradability of organic compounds at low concentrations - Part I: Shake-flask batch test with surface water or surface watedsediment suspensions*， 2002-11-15 Update
ISO 14592-2 Water quality -Evaluation of the aerobic biodegradabilityof organic compounds at low concentrations- Part 2: Continuous flow river model with attached biomass*， 2002-11-15 Update
ISO 14593 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO<(Index)2> headspace test)
ISO 7827 Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium - Method by analysis of dissolved organic carbon (DOC)*， 2010-11-15 Update
ISO 9439 Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Carbon dioxide evolution test

2001 ISO 16221:2001 Water quality - Guidance for determination of biodegradability in the marine environment