T/SDAA 0046-2021 (English Version) PDF

Scope: 3. Principle: Clostridium perfringens can secrete α-toxin and θ-toxin to cause hemolysis in blood agar culture medium, and preliminary screening of suspicious bacteria; according to Clostridium perfringens, it is a gram-positive bacillus and α-toxin can decompose egg yolk. The lecithin produces lecithinase on the egg yolk agar medium, a milky white turbid circle and the most prominent biochemical characteristics: the lactose in fermented milk causes lactic acid coagulation and produces a large amount of gas, causing the clot to burst into a porous sponge-like "burst fermentation" phenomenon , Preliminary identification of Clostridium perfringens; a pair of specific primers was designed based on the 16srDNA gene sequence of this bacterium, and a specific target gene fragment was amplified using DNA nucleic acid as a template. The size of the PCR product was determined to be 220bp by agarose gel electrophoresis. Identification of Clostridium perfringens.

T/SDAA 0046-2021 history