GB/T 41689-2022
Soil quality—Direct extraction of soil DNA (English Version)
- Standard No.
- GB/T 41689-2022
- Language
- Chinese, Available in English version
- Release Date
- 2022
- Published By
- General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
- Latest
- GB/T 41689-2022
- Scope
- This document describes a method for the direct extraction of DNA from soil samples, which is used to perform soil bacterial community abundance and composition analysis based on various molecular biology techniques, including real-time quantitative PCR (qPCR). This method is mainly applicable to agricultural and forest soils. This method may not be suitable for soils rich in organic matter (such as peat soils), soils heavily contaminated with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides a unique perspective for studying the α-diversity and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained through soil DNA PCR amplification will facilitate the development of routine tools for monitoring microbial communities in soil environments.
GB/T 41689-2022 history
- 2022 GB/T 41689-2022 Soil quality—Direct extraction of soil DNA
