ASTM E1022-22
Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks

Standard No.

ASTM E1022-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1022-22

Scope

1.1 This guide describes procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water—but not to food—by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique. These procedures also should be useful for conducting bioconcentration tests with other aquatic species, although modifications might be necessary. 1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. The comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting bioconcentration tests. 1.3 These procedures are applicable to all chemicals that can be measured accurately at the necessary concentrations in water and in appropriate tissues. Bioconcentration tests are usually conducted on individual chemicals but can be conducted on mixtures if appropriate measurements can be made. Some techniques described in this guide were developed for tests on non-ionizable organic chemicals (see 11.1.2.1) and might not apply to ionizable or inorganic chemicals. 1.4 Results of bioconcentration tests should usually be reported in terms of apparent steady-state and projected steadystate bioconcentration factors (BCFs) and uptake and depuration rate constants. Results should be reported in terms of whole body for fishes and in terms of total soft tissue for bivalve mollusks. For fishes and scallops consumed by humans, some results should also be reported in terms of the edible portion, especially if ingestion of the test material by humans is a major concern. For tests on organic and organometallic chemicals, the percent lipids of the tissue should be reported. 1.5 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Safety Precautions 7 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Metering System 6.3 Test Chambers 6.4 Cleaning 6.4.4 Acceptability 6.5 Dilution Water 8 Requirements 8.1 Source 8.2 Treatment 8.3 Characterization 8.4 Test Material 9 General 9.1 Radiolabeled Material 9.2 Stock Solution 9.3 Test Concentration(s) 9.4 Test Organisms 10 Species 10.1 Size 10.2 Source 10.3 Care and Handling 10.4 Feeding 10.5 Disease Treatment 10.6 Holding 10.7 Acclimation 10.8 Quality 10.9 Procedure 11 Experimental Design 11.1 Dissolved Oxygen 11.2 Temperature 11.3 Loading 11.4 Beginning the Test 11.5 Care of Organisms 11.6 Feeding 11.7 Cleaning 11.8 Biological Data 11.9 Measurements on Test Solutions 11.10 Analytical Methodology 12 Acceptability of Test 13 Calculation of Results 14 Documentation 15 Keywords 16 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved April 1, 2022. Published June 2022. Originally approved in 1984. Last previous edition approved in 2013 as E1022 – 94(2013) which was withdrawn January 2022 and reinstated in April 2022. DOI: 10.1520/ E1022-22. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.Specific precautionary statements are given in Section 7.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E1022-22 Referenced Document

• ASTM D1129 Standard Terminology Relating to Water
• ASTM D1193 Standard Specification for Reagent Water
• ASTM D4447 Standard Guide for Disposal of Laboratory Chemicals and Samples
• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1191 Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
• ASTM E1193 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
• ASTM E1241 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
• ASTM E1295 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
• ASTM E1688 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
• ASTM E1706 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
• ASTM E1733 Standard Guide for Use of Lighting in Laboratory Testing
• ASTM E1847 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
• ASTM E2122 Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves
• ASTM E2455 Standard Guide for Conducting Laboratory Toxicity Tests with Freshwater Mussels
• ASTM E729 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update

ASTM E1022-22 history

• 2022 ASTM E1022-22 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
• 1994 ASTM E1022-94(2013) Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
• 1994 ASTM E1022-94(2007) Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
• 2002 ASTM E1022-94(2002) Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
• 1994 ASTM E1022-94 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks