ISO 16649-3:2015/Amd 1:2016
Microbiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase- positive Escherichia coli — Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3- indolyl-ß-D-glucuronide

Standard No.

ISO 16649-3:2015/Amd 1:2016

Release Date

2016

Published By

/

Latest

ISO 16649-3:2015/Amd 1:2016

Scope

This part of ISO 16649 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is applicable to the following: — products intended for human consumption and the feeding of animals; — environmental samples in the area of food production and food handling. The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products. A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability. The method is intended to be applied and the results interpreted in the light of the information given in Clause 11. This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.

ISO 16649-3:2015/Amd 1:2016 Referenced Document

• ISO 11133 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media AMENDMENT 1
• ISO 6887-1 Microbiology of the food chain*， 2024-03-01 Update
• ISO 6887-2 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products*， 2017-03-01 Update
• ISO 6887-3 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery*， 2020-03-03 Update
• ISO 6887-4 Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 4: Specific rules for the preparation of miscellaneous products*， 2017-03-01 Update
• ISO 6887-5 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and milk pro*， 2020-04-30 Update
• ISO 6887-6 Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage
• ISO 7218 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations AMENDMENT 1

ISO 16649-3:2015/Amd 1:2016 history

• 2016 ISO 16649-3:2015/Amd 1:2016 Microbiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase- positive Escherichia coli — Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3- indolyl-ß-D-glucuronide
• 2015 ISO 16649-3:2015 Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide