T/CCAA 40-2022
Real-time fluorescent PCR method for qualitative detection of wild rice-derived components in miscellaneous grain products (English Version)

Standard No.

T/CCAA 40-2022

Language

Chinese, Available in English version

Release Date

2022

Published By

Group Standards of the People's Republic of China

Latest

T/CCAA 40-2022

Scope

Preface 1 1 Scope 2 2 Normative references 2 3 Terms, definitions and abbreviations 2 3.1 Terms and definitions 2 3.2 Abbreviations 2 4 Principle 3 5 Primers and probes for detection 3 6 Reagents and materials 3 7 Instruments and equipment 4 8 Testing steps 4 8.1 DNA extraction 4 8.2 Determination of DNA concentration and purity 4 8.3 Real-time fluorescence PCR detection 5 9 Quality control 5 10 Result judgment and expression 5 11 Result report 6 12 Anti-pollution measures 6 Appendix A (normative) reagents and their preparation 7 Appendix B (informative) Gene amplification target reference sequence of wild rice-derived components 8

T/CCAA 40-2022 history

• 2022 T/CCAA 40-2022 Real-time fluorescent PCR method for qualitative detection of wild rice-derived components in miscellaneous grain products