T/LTIA 08-2020
Technical regulation of qRT-PCR for detecting of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid (English Version)
- Standard No.
- T/LTIA 08-2020
- Language
- Chinese, Available in English version
- Release Date
- 2020
- Published By
- Group Standards of the People's Republic of China
- Latest
- T/LTIA 08-2020
- Scope
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid qRT-PCR detection uses a method that combines PCR amplification and fluorescent probes to synthesize the highly conserved sequence of severe acute respiratory syndrome coronavirus 2. Specific primers and specific fluorescent dual-labeled probes. The 5' end of the probe is labeled with a fluorescein group and the 3' end is labeled with a quenching group. During the PCR amplification process, specific primers and probes bind to the target sequence and are cut off when extending to the fluorescent probe through the DNA polymerase activity and 5'-3' exonuclease activity of Taq enzyme. When the fluorescein group and the quenching group separate, the quenching effect disappears and a fluorescent signal is generated. By detecting fluorescence signals, qualitative detection of SARS-CoV-2 nucleic acid in samples is achieved.
T/LTIA 08-2020 history
- 2020 T/LTIA 08-2020 Technical regulation of qRT-PCR for detecting of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid
