This standard specifies test objects, test requirements, sample collection, storage and transportation requirements; test steps include conditions that laboratories must meet; and DNA quality judgment standards; library construction and high-throughput sequencing processes; data information analysis and Data quality control requirements. Among them, data quality control stipulates quality requirements and quantity requirements. After the quality requirements sequencing is completed, the effective available data quality value corresponding to each DNA sample should meet the following requirements: Q20≥90%, and the base quality of the sample sequencing results is more than 90%. The value is greater than 20; Q30≥80%, more than 80% of the base quality values of the sequencing results of this sample are greater than 30. Quantity requirements: In order to complete subsequent data analysis steps such as species annotation and functional analysis, each sample is required to sequence a number of short sequences (
T/SZGIA 2-2016 history
2018T/SZGIA 2-2018 The Quality Standard of Using High-throughout Sequencing Data to Interpretate Human Whole Genome Variation
2017T/SZGIA 2-2016 Detection of environmental microorganisms based on high-throughput sequencing