ASTM E1562-22
Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids

Standard No.

ASTM E1562-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM E1562-22

Scope

1.1 This guide covers procedures for obtaining data concerning the adverse effects of a test material added to marine and estuarine waters on certain species of polychaetes during shortor long-term continuous exposure. The polychaete species used in these tests are either field collected or from laboratory cultures and exposed to varying concentrations of a toxicant in static or static-renewal conditions. These procedures may be useful for conducting toxicity tests with other species of polychaetes, although modifications might be necessary. 1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute, chronic, or life-cycle tests with other species of polychaetes. 1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications, these procedures can be used to conduct these tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, sediments/soils, oil drilling fluids, produced water from oil well production, and other types of industrial wastes. An LC50 (medial lethal concentration) may be calculated from the data generated in each acute and chronic toxicity test when multiple concentrations are tested. Growth, determined by a change in measured weight, and reproduction, as the change in total number of organisms, are used to measure the effect of a toxicant on life-cycle tests; data are analyzed statistically to indicate that concentration at which a significant difference occurs between the test solutions and control(s). 1.4 The results of dose-response acute or chronic toxicity tests with toxicants added experimentally to salt water should usually be reported in terms of an LC50 (mortality), or EC50 (medial effect concentration). The results of life-cycle toxicity tests with toxicants added experimentally to salt water should be reported as that concentration at which a statistically significant difference in the number of offspring or growth (determined by weight) is produced with reference to the control(s). 1.5 Where appropriate, this standard has been designed to be consistent with or complementary to other methods for assessing toxicity to invertebrates described in Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688. 1.6 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Acceptability 6.5 Safety Precautions 7 Dilution Water 8 Requirements 8.1 Source 8.2 Treatment 8.3 Characterization 8.4 Test Material 9 General 9.1 Stock Solution 9.2 Test Concentrations 9.3 Test Organisms 10 Species 10.1 Age 10.2 Source 10.3 Feeding 10.4 Holding 10.5 Quality 10.6 Procedure 11 Experimental Design 11.1 Acute Test 11.1.1 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Actionand is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2022. Published February 2022. Originally approved in 1994. Last previous edition approved in 2013 as E1562– 00(2013). DOI: 10.1520/E1562-22. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Chronic Test 11.1.2 Life-Cycle Test 11.1.3 Test Condition Specifications 11.2 Dissolved Oxygen 11.2.1 Temperature 11.2.2 Loading 11.2.3 Salinity 11.2.4 Light 11.2.5 Beginning the Test 11.3 Feeding 11.4 Duration of Test 11.5 Biological Data 11.6 Other Measurements 11.7 Hazards Analytical Methodology 13 Acceptability of Test 14 Calculation of Results 15 Report 16 Keywords 17 Appendixes: Neanthes arenaceodentata Appendix X1 Capitella capitata Appendix X2 Ophryotrocha diadema Appendix X3 Dinophilus gyrociliatus Appendix X4 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E1562-22 Referenced Document

• ASTM D4447 Standard Guide for Disposal of Laboratory Chemicals and Samples
• ASTM E1023 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
• ASTM E1192 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
• ASTM E1367 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
• ASTM E1391 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
• ASTM E1525 Standard Guide for Designing Biological Tests with Sediments
• ASTM E1611 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
• ASTM E1688 Standard Guide for Determination of the Bioaccumulation of Sediment-Associated Contaminants by Benthic Invertebrates
• ASTM E1706 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
• ASTM E1733 Standard Guide for Use of Lighting in Laboratory Testing
• ASTM E177 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
• ASTM E1847 Standard Practice for Statistical Analysis of Toxicity Tests Conducted Under ASTM Guidelines
• ASTM E729 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
• ASTM E943 Standard Terminology Relating to Biological Effects and Environmental Fate*， 2023-06-01 Update
• IEEE/ASTM SI 10 American National Standard for Metric Practice

ASTM E1562-22 history

• 2022 ASTM E1562-22 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
• 2000 ASTM E1562-00(2013) Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
• 2006 ASTM E1562-00(2006) Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
• 2000 ASTM E1562-00 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids