1.1 This test method describes a procedure for determining the electrophoretic mobility of proteins of molecular weight greater than 10 000 Daltons.
1.2 This test method uses automatic Electrophoretic Light Scattering (ELS) principles to determine the electrophoretic mobility.
1.3 The instrument2 simultaneously measures the Doppler shifts of scattered light at four different angles to determine the electrophoretic mobility distribution of protein particles. The mobility is expressed as µm-cm/V-s (micron-centimeter/voltsecond).
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
ASTM E1470-92(1998) history
1992ASTM E1470-92(2006) Standard Test Method for Characterization of Proteins by Electrophoretic Mobility
1998ASTM E1470-92(1998) Standard Test Method for Characterization of Proteins by Electrophoretic Mobility