ASTM F3088-22
Standard Practice for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

Standard No.

ASTM F3088-22

Release Date

2022

Published By

American Society for Testing and Materials (ASTM)

Latest

ASTM F3088-22

Scope

1.1 This practice covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells. 1.2 This practice does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces. 1.3 The cells may include adult, progenitor, or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types. 1.4 This practice does not cover methods for isolating or harvesting of cells. This practice does not cover methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this practice cover quantitative image analysis techniques. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM F3088-22 Referenced Document

• ASTM F2603 Standard Guide for Interpreting Images of Polymeric Tissue Scaffolds
• ASTM F2664 Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods
• ASTM F2739 Standard Guide for Quantifying Cell Viability and Related Attributes within Biomaterial Scaffolds
• ASTM F2944 Standard Practice for Automated Colony Forming Unit (CFU) Assays—Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture
• ASTM F2998 Guide for Using Fluorescence Microscopy to Quantify the Spread Area of Fixed Cells*， 2024-04-21 Update
• ASTM F3294 Standard Guide for Performing Quantitative Fluorescence Intensity Measurements in Cell-based Assays with Widefield Epifluorescence Microscopy

ASTM F3088-22 history

• 2022 ASTM F3088-22 Standard Practice for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions
• 2014 ASTM F3088-14 Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions