This standard specifies the method of using RBL-2H3 mast cell RTCA to evaluate the detection of allergic reactions in traditional Chinese medicine injections, and describes the corresponding methods in detail. This standard includes the reagents, instruments, cells, cell culture fluid and other materials required for the experiment, as well as the reagent preparation and other operating steps. The research content of this standard includes the detection principle of the method, specific method operation steps of cell culture, passage, cryopreservation and recovery, and RTCA detection. Use statistical analysis and processing software to perform statistical analysis on the experimental data. The experimental data are expressed as mean ± s. Compared with each administration group and the negative control group, P < 0.05 indicates that there is a significant difference, and there may be a risk of allergy. .
T/TPPA 0002-2022 history
2022T/TPPA 0002-2022 RBL-2H3 mast cell RTCA method for evaluating allergic reactions of traditional Chinese medicine injections