INTRODUCTION The measurement of DNA repair as unscheduled DNA synthesis (UDS) following chemically induced DNA damage has been shown to be a valuable tool in assessing the genotoxic activity of chemicals. Several in vitro assays for the measurement of UDS have been described (Martin et al.@ 1978; San and Stich@ 1975; Trosko and Yager@ 1974); however@ these assays often do not provide a means to metabolize chemicals to an active form. A substantial advance was made by the development of an assay that employs primary rat hepatocytes (Williams@ 1977). This assay offers many advantages over other in vitro UDS systems: (1) the low level of DNA replication in the adult rat liver (lt; 0.1%) precludes the need for inhibitors of DNA replication; (2) the same cells serve as both activator and target; and (3) a more accurate profile of metabolism is obtained than with microsomal preparations. This assay has been shown to detect a wide variety of genotoxic agents@ including polycyclic aromatic hydrocarbons@ nitrosamines@ mycotoxins@ and aromatic amines.
API PUBL 33-31751-1986 history
1986API PUBL 33-31751-1986 EVALUATION OF THE POTENTIAL OF PS-8-76D5-ARO TO INDUCE UNSCHEDULED DNA SYNTHESIS IN PRIMARY RAT HEPATOCYTE CULTURES