INTRODUCTION The purpose of this study was to evaluate the mutagenic potential of the test substance@ No.2 Home Heating Oil@ using three test systems; the Ames Test@ the Mouse Lymphoma Test and the In Vivo Bone Marrow Cytogenetics Assay. The Ames Test is bacterial mutagenicity assay utilizing selected strains of Salmonella typhimurium. The organisms are exposed to varying concentrations of the test agent both with and without metabolic activation. The Mouse Lymphoma Test is mammalian cell assay utilizing the L5178Y mouse lymphoma cell line. The cells are exposed to varying concentrations of the agent both with and without metabolic activation. The assay measures the mutation frequency at the specific locus for thymidine kinase (TK). The In vivo Bone Marrow Cytogentics assay evaluates the mutagenic potential of a test material as manifested by the production of cytogenetic abnormalities such as deletions@ exchanges@ rings@ and breaks in the chromosomes of bone marrow cells of rats receiving the test material. The following report will include the results of testing 7-1-100 utilizing the three test systems described above. The study was initiated on June 6@ 1979@ and completed November 5@ 1979.
API PUBL 28-30725-1981 history
1981API PUBL 28-30725-1981 IN VITRO AND IN VIVO MUTAGENCITY STUDIES QUENCH WATER FROM DELAYED PROCESS COKE Liquid 7-1-100