API PUBL 32-32407-1985 EVALUATION OF THE POTENTIAL OF RO-1@ 81-15@ AND PS8-76D5 SAT TO INDUCE UNSCHEDULED DNA SYNTHESIS IN PRIMARY RAT HEPATOCYTE CULTURES
INTRODUCTION The measurement of DNA repair as unscheduled DNA synthesis (UDS) following chemically induced DNA damage has been shown to be a valuable tool in assessing the genotoxic activity of chemicals. Several in vitro assays for the measurement of UDS have been described (Martin et al.@ 1978; San and Stich@ 1975; Trosko and Yager@ 1974); however@ these assays often do not provide a means to metabolize chemicals to an active form. A substantial advance was made by the development of an assay that employs primary rat hepatocytes (Williams@ 1977). This assay offers many advantages over other in vitro UDS systems: (1) the low level of DNA replication in the adult rat liver (lt; 0.1%) precludes the need for inhibitors of DNA replication; (2) the same cells serve as both activator and target; and (3) a more accurate profile of metabolism is obtained than with microsomal preparations. This assay has been shown to detect a wide variety of genotoxic agents@ including polycyclic aromatic hydrocarbons@ nitrosamines@ mycotoxins@ and aromatic amines.
API PUBL 32-32407-1985 history
1985API PUBL 32-32407-1985 EVALUATION OF THE POTENTIAL OF RO-1@ 81-15@ AND PS8-76D5 SAT TO INDUCE UNSCHEDULED DNA SYNTHESIS IN PRIMARY RAT HEPATOCYTE CULTURES